benign breast cancer cell line mcf 10a  (PromoCell)

Journal: Scientific Reports

Article Title: Effect of SSRI exposure on the proliferation rate and glucose uptake in breast and ovary cancer cell lines

doi: 10.1038/s41598-020-80850-9

Figure Lengend Snippet: SSRI treatment or 5-HT exposure marginally impact cell proliferation of breast cancer cell lines. Bar graphs depict relative proliferation assessed by Fluoroskan of MCF-10A ( a ), MCF-7 ( b ), MDA-MB-231 ( c ), MDA-MSC-hyb1 ( d ) and MDA- MSC-hyb3 ( e ) cells in response to treatment with 10 nM, 100 nM or 1000 nM fluoxetine (Fluo; yellow), sertraline (Sert; red), citalopram (Cita; blue) or 5-HT (grey) for indicated time periods normalized to DMSO-treated control cells (Ctrl; white). Data are depicted as mean ± SEM and summarize n = 9–10 (Ctrl) and at least n = 3 (SSRI/5-HT) experiments. P -values were determined by two-way ANOVA followed by Dunnett’s multiple comparison test; *** P < 0.001; ** P < 0.01; * P < 0.05 versus corresponding Ctrl.

Article Snippet: The benign breast cancer cell line MCF-10A was grown in phenol red-free Mammary Epithelial Cell Basal Medium (MECBM) with appropriate supplement mix (PromoCell, Heidelberg, Germany).

Techniques:

Journal: Scientific Reports

Article Title: Effect of SSRI exposure on the proliferation rate and glucose uptake in breast and ovary cancer cell lines

doi: 10.1038/s41598-020-80850-9

Figure Lengend Snippet: Effect of low-dose, long-term SSRI treatment or 5-HT exposure on breast cancer cell lines measured by MTT assay. Bar graphs depict relative absorbance of MTT assay of MCF-10A ( a ), MCF-7 ( b ), MDA-MB-231 ( c ), MDA-MSC-hyb1 ( d ) and MDA-MSC-hyb3 ( e ) cells in response to treatment with 100 nM or 1000 nM fluoxetine (Fluo; yellow), sertraline (Sert; red), citalopram (Cita; blue) or 5-HT (grey) for indicated time periods (96 h to 144 h) compared to DMSO-treated control cells (Ctrl; white) and cells receiving carboplatin (CP; 1000 nM; black). Data are depicted as mean ± SEM and summarize n = 3 experiments. P -values were determined by two-way ANOVA followed by Dunnett’s multiple comparison test; *** P < 0.001; ** P < 0.01; * P < 0.05 versus corresponding Ctrl.

Article Snippet: The benign breast cancer cell line MCF-10A was grown in phenol red-free Mammary Epithelial Cell Basal Medium (MECBM) with appropriate supplement mix (PromoCell, Heidelberg, Germany).

Techniques: MTT Assay

Journal: Scientific Reports

Article Title: Effect of SSRI exposure on the proliferation rate and glucose uptake in breast and ovary cancer cell lines

doi: 10.1038/s41598-020-80850-9

Figure Lengend Snippet: Fluoxetine and sertraline marginally increase glucose uptake in SK-OV-3 cells. Bar graphs depict relative cellular glucose uptake assessed by 18F-FDG incorporation of MCF-10A ( a ), MCF-7 ( b ) and MDA-MB-231 ( c ) breast cancer as well as SK-OV-3 ( d ) NIH:OVCAR-3 ( e ) and SK-MSC-hyb1 ( f ) ovarian cancer cells in response to stimulation with 1 µM fluoxetine (Fluo), sertraline (Sert), citalopram (Cita), or 5-HT relative to DMSO-treated control cells (Ctrl) for 72 h. Data, derived from n = 3 (b and e), n = 4 (a and c) or n = 5 (d and e) independent cell passages performed in duplicates, are depicted as means ± SEM. P -values were computed by one-way ANOVA followed by Dunnett’s multiple comparison test; ** P < 0.01.

Article Snippet: The benign breast cancer cell line MCF-10A was grown in phenol red-free Mammary Epithelial Cell Basal Medium (MECBM) with appropriate supplement mix (PromoCell, Heidelberg, Germany).

Techniques: Derivative Assay